A genetic dissection of the LlaJI restriction cassette reveals insights on a novel bacteriophage resistance system |
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| Authors: | Jonathan O'Driscoll Daniel F Heiter Geoffrey G Wilson Gerald F Fitzgerald Richard Roberts Douwevan Sinderen |
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| Institution: | (1) Department of Microbiology, University College Cork, Western Road, Cork, Ireland;(2) Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland;(3) Biotransfer Unit, University College Cork, Western Road, Cork, Ireland;(4) New England Biolabs, 240 County Road, 01938-2723 Ipswich, MA, USA |
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| Abstract: | Background Restriction/modification systems provide the dual function of protecting host DNA against restriction by methylation of appropriate
bases within their recognition sequences, and restriction of foreign invading un-methylated DNA, such as promiscuous plasmids
or infecting bacteriphage. The plasmid-encoded LlaJI restriction/modification system from Lactococcus lactis recognizes an asymmetric, complementary DNA sequence, consisting of 5'GACGC'3 in one strand and 5'GCGTC'3 in the other and
provides a prodigious barrier to bacteriophage infection. LlaJI is comprised of four similarly oriented genes, encoding two
5mC-MTases (M1.LlaJI and M2.LlaJI) and two subunits responsible for restriction activity (R1.LlaJI and R2.LlaJI). Here we
employ a detailed genetic analysis of the LlaJI restriction determinants in an attempt to characterize mechanistic features
of this unusual hetero-oligomeric endonuclease. |
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