Quantitative X-ray microanalysis of spleen lysosomes after acid phosphatase reaction |
| |
Authors: | G M Roomans R Wróblewski |
| |
Affiliation: | (1) Department of Ultrastructure Research, Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S-11345 Stockholm, Sweden;(2) Department of Physiology II, Karolinska Institute, Box 60400, S-10401 Stockholm, Sweden |
| |
Abstract: | Summary A quantitative X-ray microanalytical study of 80 spleen lysosomes after histochemical reaction for acid phosphatase was carried out. Lysosomal Fe concentrations showed a skewed distribution, with most lysosomes having relatively low concentrations. The concentration of Pb in the lysosomes (indicative of acid phosphatase activity) showed a close to normal distribution. Although the plot of lysosomal Pb concentrations against Fe concentrations showed a marked scattering of the points, there is a statistically highly significant correlation between accumulated metal and acid phosphatase activity as determined from the Pb concentrations. The relation between concentrations of P and those of Pb in the lysosomes points to the possibility that Pb(H2PO4)2 is the main reaction product of the histochemical acid phosphatase reaction. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|