Detection and confirmation of Clostridium botulinum in water used for cooling at a plant producing low-acid canned foods |
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Authors: | Sachdeva Amita Defibaugh-Chávez Stephanie L H Day James B Zink Donald Sharma Shashi K |
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Affiliation: | FDA, Center for Food Safety and Applied Nutrition, College Park, MD 20740, USA. |
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Abstract: | Our laboratory tested water samples used for cooling low-acid canned foods at a canning facility under investigation by the U.S. Food and Drug Administration. We used an enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies (DIG-ELISA) and real-time PCR as screening methods and confirmed the presence of neurotoxin-producing Clostridium botulinum in the samples by mouse bioassay. |
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