Biosynthesis of plant-specific flavones and flavonols in Streptomyces venezuelae |
| |
Authors: | Park Sung Ryeol Paik Ji Hye Ahn Mi Sun Park Je Won Yoon Yeo Joon |
| |
Institution: | Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea. |
| |
Abstract: | Recently, recombinant Streptomyces venezuelae has been established as a heterologous host for microbial production of flavanones and stilbenes, a class of plant-specific polyketides. In the present work, we expanded the applicability of the S. venezuelae system to the production of more diverse plant polyketides including flavones and flavonols. A plasmid with the synthetic codon-optimized flavone synthase I gene from Petroselium crispum was introduced to S. venezuelae DHS2001 bearing a deletion of the native pikromycin polyketide synthase gene, and the resulting strain generated flavones from exogenously fed flavanones. In addition, a recombinant S. venezuelae mutant expressing a codon-optimized flavanone 3beta-hydroxylase gene from Citrus siensis and a flavonol synthase gene from Citrus unshius also successfully produced flavonols. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|