Participation of valine 171 in alpha-Helix 5 of Bacillus thuringiensis Cry1Ab delta-endotoxin in translocation of toxin into Lymantria dispar midgut membranes |
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| Authors: | Alzate Oscar Osorio Cristina Florez Alvaro M Dean Donald H |
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| Affiliation: | Biochemistry Department, The Ohio State University, 484 W. 12th Avenue, Columbus, Ohio 43210, USA. |
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| Abstract: | The Cry1Ab δ-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar, which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta, another Cry1Ab δ-endotoxin-susceptible insect. |
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