P、Zn在小麦细胞内的积累、分布及交互作用的研究

报道了小麦无性系细胞在含有3个P水平(0.5、1.5、3.5mmol·L^-1)和2个Zn水平(0、60μmol·L^-1)的M.S培养液中,细胞对P、Zn的积累、分布特性及交互作用的研究.P和Zn在细胞内的积累分别随外施P、Zn浓 度的提高而提高.与缺Zn比较,加Zn处理能抑制培养7d细胞中P的含量.营养液中0.5～1.5mmol·L^-1 P能促进细胞的含Zn量,但高P处理即抑制细胞的含Zn量.小麦细胞壁固定了细胞83.9%～88.3%的P,而外施P浓度越高,则细胞壁中P分配的比例越大.在供Zn条件下,细胞壁截留了细胞中一半以上的Zn(52.0～60.5%).小麦液泡中P和Zn的含量较少,分别为2.2%～3.8%和10.6%～30%.

Accumulation,distribution and interaction of phosphorus and zinc in wheat cells

This paper studied the accumulation,distribution and interaction of phosphorus and zinc in the asexual cells of wheat cultured in MS solution with three levels of phosphorus(0.5,1.5,3.5 mmol·L^-1) and two levels of zinc(0,60 μmol·L^-1).An application of 60μmol·L^-1 Zn and 0.5～1.5mmol·L^-1 P increased the reproduction and the dry matter yield of wheat cells,while the supply with no Zn or high P(3.5 mmol·L^-1) showed an inhibited effect.The accumulation of P and Zn in cells increased with increasing P and Zn supply. In comparison with Zn-deficiency,an application of 60μmol·L^-1 Zn reduced the P content in cells cultured for 7 days. 0.5～1.5 mmol·L^-1 P enhanced Zn accumulation in cells, but decreased it under 3.5 mmol·L^-1 P. As much as 83.9%～88.3% of the total P was associated with thecell walls, and the more the P supply,the greater the P content in them.The results also showed more than half of Zn (52.0～60.5％)in cells was examined in cell walls.In contrast to the cell walls,the contents of P and Zn in vacuoles were much lower,with 2.3～3.8% and 10.6～30% respectively.