| 以胰岛素样生长因子受体1基因为靶的反义寡核苷酸体内外抗肿瘤研究 |
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| 引用本文： | 林莉,王升启,管伟,杨秉呼,胡小电.以胰岛素样生长因子受体1基因为靶的反义寡核苷酸体内外抗肿瘤研究[J].生物化学与生物物理进展,2002,29(2):247-251. |
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| 作者姓名： | 林莉 王升启 管伟 杨秉呼 胡小电 |
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| 作者单位： | 1. 军事医学科学院附属医院,北京,100039
2. 军事医学,科学院放射医学研究所,北京,100850 |
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| 基金项目： | 国家重点基础研究发展规划项目(G1998051118)和国家高技术“863”计划资助项目(102-08-04-01). |
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| 摘 要： | 以胰岛素样生长因子受体－1基因为靶筛选抗肿瘤药物.根据IGF1RmRNA的二级结构设计了9条反义寡核苷酸药物，以脂质体介导进行转染，MTT染色计算细胞生长抑制率，从中筛选出一条序列并对之进行优化，最后以最佳序列进行体外作用持续时间及体内细胞生长抑制率分析.结果表明该序列在体内外具有良好的抗肿瘤活性，具有剂量依赖性关系，且对荷瘤裸鼠无明显的毒性.IGF1R可作为肿瘤治疗的新靶点.
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| 关 键 词： | IGF1R，反义寡核苷酸，肿瘤 |
| 收稿时间： | 6/4/2001 12:00:00 AM |
| 修稿时间： | 2001年6月4日 |
Antitumor Activity of Antisense Oligonucleotides Targeted to IGF1R in vivo and In vitro |
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| LIN Li,WANG Sheng-Qi,GUAN Wei,YANG Bing-Hu and HU Xiao-Dian.Antitumor Activity of Antisense Oligonucleotides Targeted to IGF1R in vivo and In vitro[J].Progress In Biochemistry and Biophysics,2002,29(2):247-251. |
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| Authors: | LIN Li WANG Sheng-Qi GUAN Wei YANG Bing-Hu and HU Xiao-Dian |
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| Institution: | Department of Oncology, The Affiliated Hospital, Academy of Military Medical Science, Beijing 100039, China;Beijing Institute of Radiation Medicine,Beijing 100850, China;Beijing Institute of Radiation Medicine,Beijing 100850, China;Beijing Institute of Radiation Medicine,Beijing 100850, China;Department of Oncology, The Affiliated Hospital, Academy of Military Medical Science, Beijing 100039, China |
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| Abstract: | In order to Screening antitumor drugs tartgeted to IGF1R gene, 9 different 20-mer anti-sensitive oligo-deoxyribonucleic acid (ASODN) were designed according to the mRNA second structure of IGF1R gene and were transfected into tumor cells under various conditions in the presence of lipofectin. Cell growth activity were evaluated by MTT assay. The best sequence with antitumor activity in vitro and in vivo were analyzed. This sequence showed strong anticancer activity in vitro and in vivo and had dose -dependent relation. The sequence had no obvious toxicity on tumor -burdended nude either . IGF1R could be used as an appropriate target for tumor therapay. |
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| Keywords: | IGF1R antisense oligonucleotides tumor |
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