| 番茄烟粉虱传双生病毒PCR检测 |
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| 引用本文： | 何自福,虞皓,罗方芳.番茄烟粉虱传双生病毒PCR检测[J].Virologica Sinica,2004,19(1):67-69. |
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| 作者姓名： | 何自福 虞皓 罗方芳 |
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| 作者单位： | 广东省农业科学院植物保护研究所 广东广州510640
(何自福，虞皓)，广东省农业科学院植物保护研究所 广东广州510640(罗方芳) |
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| 基金项目： | 广东省自然科学基金(031992)，广东省农业科学院“十五”重点科技项目(2001-基金-16) |
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| 摘 要： | From the conserved regions of the reported nucleotide sequences of whitefly-transmitted geminiviruses (WTGV), a pair of degenerate primers was designed to anneal to the conserved sequence.The tomato samples infected geminivirus-like from Guangdong were detected by PCR. The results showed that a 356bp specific fragment was amplified from the samples. The specific fragment was cloned and sequenced, and the sequence was compared with all nucleotide sequences in GenBank by Blast of NCBI. The result showed that the fragment belonged to Geminiviridae DNA. So the degenerate primers may be used to detect the WTGV from tomato in Guangdong. Moreover, both of the homology of the fragment between WTGV from tomato in Guangdong and the reported WTGV in the world and WTGV from tomato in Guangxi were under 82%. These results implied that the WTGV from tomato in Guangdong differed from the above-mentioned WTGV.
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| 关 键 词： | 烟粉虱传双生病毒 PCR检测 宁列分析 植物病毒 |
Detection of Whitefly-transmitted Geminiviruses from Tomato by PCR |
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| HE Zi-fu,YU Hao,LUO Fang-fang.Detection of Whitefly-transmitted Geminiviruses from Tomato by PCR[J].中国病毒学(英文版),2004,19(1):67-69. |
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| Authors: | HE Zi-fu YU Hao LUO Fang-fang |
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| Institution: | HE Zi-fu**,YU Hao,LUO Fang-fang |
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| Abstract: | From the conserved regions of the reported nucleotide sequences of whitefly-transmitted geminiviruses (WTGV), a pair of degenerate primers was designed to anneal to the conserved sequence. The tomato samples infected geminivirus-like from Guangdong were detected by PCR. The results showed that a 356bp specific fragment was amplified from the samples. The specific fragment was cloned and sequenced, and the sequence was compared with all nucleotide sequences in GenBank by Blast of NCBI. The result showed that the fragment belonged to Geminiviridae DNA. So the degenerate primers may be used to detect the WTGV from tomato in Guangdong. Moreover, both of the homology of the fragment between WTGV from tomato in Guangdong and the reported WTGV in the world and WTGV from tomato in Guangxi were under 82%. These results implied that the WTGV from tomato in Guangdong differed from the above-mentioned WTGV. |
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| Keywords: | Whitefly-transmitted geminivirus(WTGV) PCR detection Sequence analysis |
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