| 凋亡而不出现DNA梯形带一例 |
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| 引用本文： | 胡庆柳
,丁振华
,谭小华.凋亡而不出现DNA梯形带一例[J].细胞生物学杂志,1998(2). |
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| 作者姓名： | 胡庆柳 丁振华 谭小华 |
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| 作者单位： | 第一军医大学基础部放射医学教研室，南方医院消化科 广州 510515 |
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| 基金项目： | 国家自然科学基金资助项目(№.39670192) |
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| 摘 要： | 用普通琼脂糖凝胶电泳UVB照射后分别培养24、36小时的NIH3T3细胞DNA,均未出现梯形带,但从细胞形态上看,大部分细胞发生凋亡并出现凋亡小体。电泳UVB照射后培养24小时的昆明小鼠胸腺细胞DNA,出现典型的凋亡梯形带。说明细胞在发生凋亡时DNA并不总是从核小体之间断裂的,不能把DNA梯形带作为判断细胞凋亡的唯一标准。
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| 关 键 词： | DNA梯形带 UVB凋亡 |
AN EXAMPLE OF APOPTOSIS WITHOUT DNA LADDERS |
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| HU Qing Liu DNG Zhen Hua TAN Xiao Hua.AN EXAMPLE OF APOPTOSIS WITHOUT DNA LADDERS[J].Chinese Journal of Cell Biology,1998(2). |
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| Authors: | HU Qing Liu DNG Zhen Hua TAN Xiao Hua |
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| Institution: | HU Qing Liu D1NG Zhen Hua TAN Xiao Hua |
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| Abstract: | When conventional gel electrophoresis was used,it was found that DNA of NIH3T3 cells cultured for 24,36 hours after UVB irradiation had no DNA ladders,but tnorphologicaly,the majority were apoptotic and had apoptotic bodies emerged. Thymocytes of Kunming mouse processed by the same method had DNA ladders. It is suggested that DNA cleavage of apoptotic cell is not always initiate at internucleosome. DNA ladders should not be the sole criterion for identifying apoptosis. |
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| Keywords: | DNA ladder UVB apoptosis |
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