Receptors for agglutinin: New cell surface markers on a spontaneous leukemia |
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Authors: | Takashi Muramatsu Hisako Muramatsu Masataka Kasai Sonoko Habu Ko Okumura |
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Affiliation: | 1. Department of Biochemistry, Kagoshima University School of Medicine, Usukicho, Kagoshima, Japan;2. Department of Biochemistry, Kobe University School of Medicine, Kobe, Japan;3. Department of Tuberculosis, National Institute of Health, Tokyo, Japan;4. Department of Pathology, School of Medicine, Tokai University, Kanagawa, Japan;5. Department of Immunology, University of Tokyo, Tokyo, Japan. |
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Abstract: | agglutinin (DBA), which is specific for terminal α-N-acetylgalactosamine, bound to a spontaneous leukemia cell of mice, but not to lymphoid cells of the host. The DBA receptors were isolated from the leukemia cell labeled with [3H]-galactose after detergent solubilization and affinity chromatography on DBA-agarose. The major component of the receptors migrated as a glycoprotein of apparent molecular weight 100,000 upon SDS gel electrophoresis. Alkaline treatment degraded the glycoproteins, releasing oligosaccharides of molecular weight around 1,000. |
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Keywords: | DBA EC embryonal carcinoma FITC fluorescent isothiocyanate SDS sodium dodecyl sulfate |
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