Receptors for <span class="math"><math><mtext>Dolichos biflorus</mtext></math></span> agglutinin: New cell surface markers on a spontaneous leukemia期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Receptors for $Dolichos biflorus$ agglutinin: New cell surface markers on a spontaneous leukemia

Authors:	Takashi Muramatsu Hisako Muramatsu Masataka Kasai Sonoko Habu Ko Okumura

Affiliation:	1. Department of Biochemistry, Kagoshima University School of Medicine, Usukicho, Kagoshima, Japan;2. Department of Biochemistry, Kobe University School of Medicine, Kobe, Japan;3. Department of Tuberculosis, National Institute of Health, Tokyo, Japan;4. Department of Pathology, School of Medicine, Tokai University, Kanagawa, Japan;5. Department of Immunology, University of Tokyo, Tokyo, Japan.

Abstract:	$Dolichos biflorus$ agglutinin (DBA), which is specific for terminal α-N-acetylgalactosamine, bound to a spontaneous leukemia cell of $GRS A$ mice, but not to lymphoid cells of the host. The DBA receptors were isolated from the leukemia cell labeled with [³H]-galactose after detergent solubilization and affinity chromatography on DBA-agarose. The major component of the receptors migrated as a glycoprotein of apparent molecular weight 100,000 upon SDS gel electrophoresis. Alkaline treatment degraded the glycoproteins, releasing oligosaccharides of molecular weight around 1,000.

Keywords:	DBA EC embryonal carcinoma FITC fluorescent isothiocyanate SDS sodium dodecyl sulfate
本文献已被 ScienceDirect 等数据库收录！