Demonstration of complexity of the glycosphingolipid fraction of rat small intestine |
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Authors: | Michael E. Breimer Gunnar C. Hansson Karl-Anders Karlsson Hakon Leffler |
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Affiliation: | Department of Medical Biochemistry, University of Göteborg, Box 33031, S-400 33 Göteborg, Sweden |
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Abstract: | A total neutral (non-acid) glycolipid fraction has been isolated from rat small intestine. By silicic acid column chromatography of the acetylated glycolipid derivative, 7 different partly purified fractions were obtained. Thin-layer chromatography of both the acetylated and native glycolipid fractions revealed a highly complex pattern with at least 30 different glycolipid bands having a thin-layer mobility as for mono- to dodecaglycosylceramides. Mass spectrometry of the permethylated and permethylated-reduced (LiAlH4) derivatives showed the presence of several glycolipid species not known before, including olighexosylceramides with 4, 5, 6 and 7 sugar residues and a tetraglycosylceramide with a blood group A determinant. This is the first report on such a complex glycolipid composition of a single organ. |
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