Role of nonenzymatic glycosylation in experimental cataract formation |
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Authors: | Shyh-Horng Chiou Leo T Chylack HFranklin Bunn Jin H Kinoshita |
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Institution: | 1. Howard Hughes Medical Institute Laboratory, Peter Bent Brigham Hospital, Harvard Medical School, Boston, Mass. 02115 USA;1. Laboratory for Vision Research, National Eye Institute, NIH, Bethesda, Maryland, USA |
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Abstract: | The relevance of nonenzymatic glycosylation of lens proteins to cataract formation was studied in rats on a normal and high galactose diet, treated with and without sorbinil, an aldose reductase inhibitor. All galactosemic rats not receiving sorbinil had cataracts; none receiving sorbinil had cataracts. Lens homogenate was treated with a 200 fold molar excess of 3H]-borohydride and the extent of glycosylation was estimated from radioactivity incorporation and quantitation of hexitol-lysine adduct after extensive dialysis. We found no differences in the radioactivity uptake nor the amounts of hexitol-lysine in the lenses of galactosemic rats treated with and without sorbinil. Thus, nonenzymatic glycosylation was not responsible for the sugar-induced cataracts. |
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