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Mobilization of arachidonic acid from phosphatidylethanolamine fraction to phosphatidylcholine fraction in platelets |
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| Authors: | Reiji Kannagi Kinya Koizumi Sachiko Hata-Tanoue Tohru Masuda |
| Institution: | 1. Department of Pathology Faculty of Medicine, Kyoto University, Kyoto, Japan.;2. Laboratory for Clinical Investigation Faculty of Medicine, Kyoto University, Kyoto, Japan.;3. Institute of Immunology, Faculty of Medicine, Kyoto University, Kyoto, Japan.;4. Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Nagoya, Japan. |
| Abstract: | Rabbit platelets rapidly incorporated methyl groups of 3H] methionine to phosphatidylcholine (PC). Rabbit platelets also incorporated 3H]choline to PC, but the rate of incorporation was far lower than that of 3H]methionine. Further fractionation of labeled PC revealed that a considerable amount of arachidonyl PC was synthesized via the N-methylation pathway. Thrombin stimulation resulted in a release of arachidonic acid from PC, and not from phosphatidylethanolamine (PE). These observations suggest that the N-methylation pathway plays an important role in the intracellular mobilization of arachidonic acid from the PE fraction to the PC fraction, this fraction being more sensitive to the hydrolysis with phospholipase A2 during platelet activation. |
| Keywords: | AA arachidonic acid PC phosphatidylcholine PE phosphatidylethanolamine PI phosphatidylinositol PMME phosphatidylmonomethylethanolamine PDME phosphatidyldimethylethanolamine TBS Tris-buffered saline |
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