The relative contribution of somatomedin to the serum-stimulated growth of human fibroblasts |
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Authors: | Ariane Plet Yoheved Berwald-Netter |
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Institution: | 1. Laboratoire de Recherches sur la Croissance, INSERM FRA 31 74, Avenue Denfert-Rochereau, 75014 Paris, France;2. Biochimie Cellulaire, Collège de France, 75231 Paris Cedex 05, France |
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Abstract: | Culture conditions were defined allowing to demonstrate a stimulatory effect of both serum-contained and purified Somatomedin activity on incorporation of 3H]thymidine and replication of cultured normal human fibroblasts. The use of dialyzed human serum in MEM medium supplemented by 0.2 mM serine offered the necessary and sufficient culture conditions. A significant difference between normal and hypopituitary patients sera was found in their effect on the rate of 3H]thymidine incorporation (p < 0.0001) and on cell replication (p < 0.01). Purified Somatomedin-C, in MEM without serum, is a poor mitogen. Its activity was strongly enhanced by the addition of 0.1 % dialyzed serum and 0.2 mM serine without, however, exceeding the stimulatory level of 1 % whole normal serum. The requirement of concomitant presence, for optimal cell growth, of different low and high MW serum components is discussed. |
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Keywords: | BSA bovine serum albumin GH growth hormone MEM minimal Eagle's medium MSA multiplication stimulating activity NSILA non suppressible insulin-like activity SM Somatomedin SM-C Somatomedin-C [3H]Td tritiated thymidine |
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