Effect of hemopexin on the efflux of metalloporphyrin from isolated rat liver mitochondria |
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Authors: | Paul Husby Ursula Műller-Eberhard Inge Romslo |
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Institution: | 1. Laboratory of Clinical Biochemistry, University of Bergen, N-5016 Haukeland Sykehus, Norway.;2. Department of Biochemistry, Scripps Clinic and Research Foundation, La Jolla, CA 92037, U.S.A. |
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Abstract: | When rat liver mitochondria were incubated with 57Co2+ and deuteroporphyrin (or protoporphyrin) the efflux of metalloporphyrin was markedly increased when hemopexin was included. The effect of hemopexin could be abolished by adding hemin, or in part by high concentrations of K+. Globin behaved essentially as hemopexin. The results could not be ascribed to the removal of metalloporphyrin from de-energized, leaky mitochondria. The results are strong evidence for a protein-facilitated transport of metalloporphyrin from the mitochondria to the cytosol. |
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Keywords: | HEPES N-2-hydroxyethyl-piperazine-N′-2-ethanesulphonic acid |
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