Transformation of Saccharomyces cerevisiae with yeast mitochondrial DNA linked to two micron circular yeast plasmid |
| |
| Authors: | Bentley A. Atchison Rodney J. Devenish Anthony W. Linnane Phillip Nagley |
| |
| Affiliation: | Department of Biochemistry, Monash University, Clayton, Victoria, 3168, Australia |
| |
| Abstract: | Mitochondrial DNA from a petite mutant of yeast carrying an oligomycin resistance determinant has been ligated in vitro to 2 μm yeast plasmid DNA. The recombinant DNA so produced has been used to transform an oligomycin sensitive strain of Saccharomyces cerevisiae to oligomycin resistance at a frequency approaching 50 times the spontaneous mutation rate to oligomycin resistance. The majority of transformants showed genetic properties suggesting that recombination between the transforming DNA and the resident mtDNA has occurred. The properties of a subclass of oligomycin resistance transformants suggested that in these cells the transforming DNA has not become stably integrated into the mitochondrial genome of the recipient cell. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
