Increase in acetylation of spermidine in rat liver extracts brought about by treatment with carbon tetrachloride

Within three hours of the administration of hepatotoxic doses of carbon tetrachloride to rats there was a substantial increase in the ability of liver extracts to catalyze the accumulation of monoacetylspermidine when incubated with spermidine and acetyl-CoA. This increase was maximal by six hours and correlated with the period in which there was a pronounced fall in hepatic spermidine and concomitant increase in putrescine. During this time there is a large increase of the conversion of labeled spermidine into putrescine in the liver. These results therefore suggest that this conversion requires the prior acetylation of spermidine.