Equilibrium centrifugation of measles virus |
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Authors: | G. Huybreghts D.A. Vanden Berghe S.R. Pattyn |
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Affiliation: | Laboratory of medical microbiology, University of Antwerp, B-2610 Wilrijk, Belgium |
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Abstract: | Measles virus has been centrifuged on different density gradients. It sediments at densities of 1,20 g/cm3 in K-tartrate, of 1,18–1,21 g/cm3 in sucrose, 1,19–1,23 g/cm3 in CsCl and 1,19 g/cm3 in metrizamide gradients. Metrizamide reduced measles virus infectivity. In sucrose gradients sometimes more than one infectious peak was observed. Control Vero cells produced particles of the same densities as measles virus peaks. These peaks did contain actin as the major protein. The relevance of this finding in relation to the presence of actin in measles virus is discussed. |
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Keywords: | RSB reticulocyte suspension buffer SDS sodium dodecyl sulfate MM maintenance medium |
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