Presence of cytochrome P-450- and cytochrome P-448-dependent monooxygenase functions in hepatoma cell lines |
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Authors: | FJ Wiebel T Wolff M Lambiotte |
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Institution: | 1. Gesellschaft f. Strahlen- u. Umweltforschung, Abteilung Toxikologie, Institut f. Toxikologie u. Biochemie, D-8042 Neuherberg b. München, Germany;2. Department Unité de Génétique Cellulaire, Institut de Recherches en Biologie Moleculaire du C.N.R.S. Université Paris VII, Place Jussieu - 75221 Paris Cedex 05, France |
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Abstract: | Cell lines derived from Reuber H-4-II-E hepatoma cells and their hybrids that differ in the expression of liver-specific functions are shown to contain different forms of monooxygenases. According to 1) the specificity toward the substrates benzo(a)pyrene, aldrin and chenodexycholic acid, 2) the kinetics of the epoxidation of aldrin, 3) the response to inducers, such as benz(a)anthracene and dexamethasone, and 4) the modifier 7,8-benzoflavone, the monooxygenases predominating in differentiated cell lines belong to the cytochrome P-450-dependent enzyme(s), those in the less differentiated lines belong to the cytochrome P-448-dependent form(s). |
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