Citrate transport in liposomes reconstituted with Triton extracts from mitochondria |
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Authors: | I Stipani R Krämer F Palmieri M Klingenberg |
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Institution: | 1. Institute of Biochemistry, University of Bari, 70126 Bari, Italy;2. Institute of Physical Biochemistry, University of Munich, 8000 Munich 2, F.R.G. |
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Abstract: | The exchange between external 14C] citrate and internal citrate, malate or phosphoenopyruvate can be reconstituted with a Triton extract of submitochondrial particles from rat liver. The reconstituted activity is dependent on the phospholipid composition of the liposomes and is influenced by the simultaneously incorporated Triton. The kinetic properties, the substrate and tissue specificity, and the inhibitor sensitivity of citrate transport in liposomes are similar to those described for the tricarboxylate transport in mitochondria. The maximal rate of citrate exchange in the reconstituted system (13.5 μmol × min?1 × g?1 at 25°C and pH 7.5) accounts for 12% of the original mitochondrial activity. |
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Keywords: | SMP submitochondrial particles EYPL egg yolk phospholipids MPL mitochondrial phospholipids BTA benzene-tricarboxylic acid PEP phosphoenolpyruvate MOPS morpholinopropane sulfonic acid |
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