Activation of brain phenylethanolamine-N-methyltransferase by Triton-X-100: Time related differences in the enzyme activation |
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Authors: | S Chaudhuri H Parvez S Parvez |
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Institution: | Unité de Neuropharmacologie, Bât. 440, Université de Paris XI, Centre d''Orsay, 91405 Orsay, France |
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Abstract: | The effect of different concentrations of Triton-X-100 (0.2 to 5 %) on the activity of enzyme phenylethanolamine-N-methyltransferase (PNMT) in the brain and adrenal was studied. The addition of 0.2 % Triton-X-100 to the 0.9 % KCl homogenization media resulted in 180 % activation of the brain PNMT. The similar content of this detergent added to the adrenal PNMT preparation had no marked effect on enzyme activity. Rising Triton-X-100 concentrations from 0.2 % to 5 % resulted in higher activation of brain PNMT activity but the adrenal enzyme remained rather stable. An exposure of 15 minutes of brain PNMT preparation to Triton-X-100 was the optimal interval to evoke the maximal increase in enzyme activity. This activation of brain PNMT by Triton-X-100 was observable up to 24 hours after the addition of the detergent. |
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