Enzymatic isomerization (Δ7 → Δ8) of the nuclear double bond of 14α-alkyl substituted sterol precursord of cholesterol |
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Authors: | Robert A Pascal George J Schroepfer |
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Institution: | 1. Department of Biochemistry Rice University, Houston, Texas 77001 USA;2. Department of Chemistry Rice University, Houston, Texas 77001 USA |
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Abstract: | The catalysis by rat liver microsomes under anaerobic conditions, of the conversion of 3α-3H]14α-methyl-5α-cholest-7-en-3β-ol and of 2,4-3H]14α-hydroxymethyl-5α-cholest-7-en-3β-ol to labeled 14α-methyl-5α-cholest-8-en-3β-ol and 14α-hydroxymethyl-5α-cholest-8-en-3β-ol, respectively, has been demonstrated. This finding is of importance in evaluating past research in this area and in consideration of pathways and mechanisms involved in enzymatic removal of carbon atom 32 of 14α-methyl sterols. Also described herein are syntheses of 2,4-3H]14α-hydroxymethyl-5α-cholest-7-en-3β-ol and 3β-acetoxy-14α-methyl-5α-cholest-8-ene. |
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Keywords: | To whom inquiries should be directed |
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