Loss of glucocorticoid regulation of plasminogen activator activity in anucleate rat hepatoma cells

Glucocorticoids decrease the plasminogen activator activity of rat hepatoma cells through production of an inhibitor. We have examined the dexamethasone regulation of plasminogen activator in anucleate rat hepatoma cells to investigate the role of the nucleus in the steroid regulation of this membrane-associated phenomenon. Dexamethasone did not affect either the intra- or extra-cellular plasminogen activator activity of the anucleate cells, and did not induce production of an inhibitor of plasminogen activator. Therefore, glucocorticoid regulation of plasminogen activator activity requires the presence of an intact nucleus.