125I-Transfer catalysed by microsomes |
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| Authors: | Gerhard Heinrich |
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| Institution: | 1. Department of Biochemistry University of Wisconsin Madison, WI 53792 USA;2. Department of Medicine University of Wisconsin Madison, WI 53792 USA |
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| Abstract: | 125I-mono-iodotyrosine and 125I-albumin were identified following protease assays with rat brain microsomes when 125I-proinsulin was the substrate. Polyacrylamide gel electrophoresis and Sephacryl 200 gel filtration under dissociating conditions suggest that these products arise through 125I-transfer rather than adsorption of hydrolytic fragments onto albumin. Similar results were obtained with insulin receptor assays on a crude rat brain membrane fraction. The transfer was inhibited by excess unlabeled substrate, but not by excess iodide. Of five subcellular fractions, only the microsome enriched fraction catalysed the transfer. |
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| Keywords: | To whom correspondence should be addressed |
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