Isolation and identification of 25-hydroxy-24-oxocholecalciferol: A metabolite of 25-hydroxycholecalciferol |
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Authors: | Y Takasaki N Horiuchi N Takahashi E Abe T Shinki T Suda S Yamada H Takayama H Horikawa T Masumura M Sugahara |
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Institution: | Department of Biochemistry, Showa University Dental School 1-5-8, Hatanodai, Shinagawa, Tokyo 142, Japan.;Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan.;Research Laboratories of Amino Feed Co. Inc., 919 Aoki, Kuroiso, Tochigi 325-01, Japan. |
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Abstract: | A metabolite of 25-hydroxycholecalciferol has been isolated in pure form from chicken kidney homogenates. It has been identified as 25-hydroxy-24-oxocholecalciferol by means of ultraviolet absorption spectrophotometry, mass spectrometry, infrared spectrometry, nuclear magnetic resonance spectrometry, and specific chemical reactions. |
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Keywords: | To whom all correspondence should be addressed |
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