Comparison of the human,canine and swine E apoproteins |
| |
Authors: | Karl H. Weisgraber Robert F. Troxler Stanley C. Rall Robert W. Mahley |
| |
Affiliation: | 1. Gladstone Foundation Laboratories for Cardiovascular Disease University of California, San Francisco, P. O. Box 40608 San Francisco, California 94140 USA;2. Department of Biochemistry School of Medicine Boston University Medical Center, Boston, Massachusetts 02118 USA |
| |
Abstract: | A comparative study of the E apoprotein isolated from the d<1.02 lipoproteins of human, canine and swine plasma revealed that the various apo-E preparations had similar molecular weights (37,000–39,000) and had similar amino acid compositions in that glutamic acid, alanine, leucine and arginine were present in high concentrations. The various preparations showed partial immunochemical cross-reactivity, demonstrating significant sequence homology between the species. However, determination of the amino-terminal amino acid sequence by automated Edman degradation showed each apo-E was different, demonstrating that the amino-terminal portion of the E apoprotein was a variable region of this protein. |
| |
Keywords: | Correspondence should be addressed to this author. |
本文献已被 ScienceDirect 等数据库收录! |
|