Bacillus thuringiensis parasporal crystal toxin: Dissociation into toxic low molecular weight peptides |
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| Authors: | PG Fast WG Martin |
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| Institution: | 1. Canadian Forestry Service Forest Pest Management Institute Sault Ste. Marie, Ontario P6A 5M7 Canada;2. National Research Council Sussex Drive Ottawa, Ontario Canada |
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| Abstract: | Parasporal crystals of Bacillus thuringiensis can be dissociated into low molecular weight peptides (< 5000 daltons) by dissolving them in 0.1 M N-morpholinopropane sulfonic acid buffer pH 7.8 containing 0.05 M dithiothreitol and 2M–4M KSCN, or by performic acid oxidation. The peptides obtained by dissolving in KSCN were still toxic to silkworm larvae. |
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