Alterations in lipid turnover in developing muscle |
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Authors: | Alexander Sandra Victor V Ionasescu |
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Institution: | 1. Department of Anatomy School of Medicine University of Iowa, Iowa City, Iowa 52242 USA;2. Department of Pediatrics School of Medicine University of Iowa, Iowa City, Iowa 52242 USA |
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Abstract: | The incorporation and turnover of 3H] glycerol into skeletal muscle cell cultures derived from embryonic chickens was studied. Both rates of incorporation and turnover of specific lipids were dependent on culture age and lipid species. The pattern of glycerol incorporation showed that prefusion myoblasts primarily synthesized both phosphatidylcholine and triglycerides whereas postfusion myotubes primarily synthesized phosphatidyl choline. This pattern could be modified in postfusion but not prefusion cells by briefly incubating the cells with unilammelar phosphatidyl choline vesicles. Analysis of major lipid species revealed that muscle triglycerides and phospholipids turned over at a higher rate in prefusion cultures compared to the postfusion state. These findings are discussed in light of the marked shift in lipid metabolism which occurs during myogenesis. |
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Keywords: | PC phosphatidylcholine PE phosphatidyl ethanolamine PS phosphatidyl serine PI phosphatidyl inositol |
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