Purification and evidence for heterogeneity of acid phosphatase from |
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Authors: | Slobodan Barbarié Branko Kozulié Blanka Ries Pavao Mildner |
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Institution: | Laboratory of Biochemistry, Faculty of Technology, University of Zagreb, Pierottijeva 6, 41000 Zagreb, Yugoslavia |
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Abstract: | The protoplast-secreted acid phosphatase of yeast was purified about 60 fold by ultrafiltration, gel filtration and chromatography on DEAE-Sephadex A-25. It was established that the enzyme is free of inactive proteins as well as polysaccharides and contains 48% of neutral sugars. The failure to separate the protein from the carbohydrates by several procedures indicates that the carbohydrate part is covalently linked to the protein. A pronounced heterogeneity of the enzyme with respect to charge as well as to molecular weight was found. The data obtained by gel filtration indicated enzyme heterogeneity in respect to carbohydrate content. |
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