Abstract: | Dithiothreitol (DTT), a disulfide reducing agent, diminished the specific binding of 3H] dopamine to partially purified calf striatal membranes (P2) but did not have an effect on 3H] spiroperidol binding. The thiol reagents, p-chloromercuribenzoate (PCMB), N-ethylmaleimide (NEM) and iodoacetamide (IA), were also tested for inhibitory effects on agonist and antagonist binding to the dopamine receptor. PCMB inhibited both 3H] dopamine and 3H] spiroperidol binding by changing the affinity (Kd) and the number of binding sites (Bmax) for both of these ligands. This effect of PCMB was reversed by the addition of DTT. NEM inhibited binding to the dopamine agonist site but not to the antagonist site, while IA was ineffective on either site. These results indicate that a DTT-reducible disulfide bond may be an essential component for agonist binding to the dopamine receptor. Furthermore, the experiments with PCMB, NEM and IA suggest that the exposure of thiol groups in the dopamine receptor may play an important role in agonist and antagonist binding. |