Metyrapone - reduced cytochrome P-450 complex: A specific method for the determination of the phenobarbital inducible form of rat hepatic microsomal cytochrome P-450 |
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| Authors: | V Luu-The J Cumps P Dumont |
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| Institution: | Department of Medicinal Chemistry Faculty of Medecine Catholic University of Louvain Emmanuel Mounier av. 73 1200 Bruxelles, Belgium |
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| Abstract: | Using homogeneous cytochrome P-450, we have shown that the well-known metyrapone-dithionite reduced cytochrome P-450 complex is specific for the cytochrome P-450b induced by phenobarbital. A linear relationship was observed between the absorbance of metyrapone-reduced cytochrome P-450 complex and the one of CO-reduced cytochrome P-450 complex, the usual method for the determination of cytochrome P-450. A method has been proposed for the specific determination of the cytochrome P-450b. |
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