Ratio of Na:K transport in reconstituted sodium pump vesicles |
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Authors: | Beatrice M. Anner |
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Affiliation: | Department of Pharmacology, University of Geneva Medical School, CH-1211 Geneva 4, Switzerland |
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Abstract: | The effect of ATP on the kinetics of Na and K fluxes across the membranes of reconstituted sodium pump vesicles was examined. In the absence of ATP, the active vesicles equilibrated with 42K or 86Rb within 6 hours. In contrast, the equilibration of intravesicular Na with external 22Na was about 4 times slower. In the presence of ATP, the intravesicular K was replaced within 3 min by Na a Na:K exchange process. The total intravesicular Na pool was then labeled to the same specific radioactivity as the Na of the medium a Na:Na exchange process. The Na:K transport ratio varied with the intravesicular concentrations of Na and K. |
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