NADH-dependent glutamate synthase and nitrogen metabolism in |
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Authors: | Gisela Hummelt Jaime Mora |
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Institution: | Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, U.N.A.M., México 20, D. F. |
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Abstract: | The GDH (NADPH) mutant strain of has sizable pools of glutamine and glutamate under ammonium-limited conditions for which requires an elevated glutamine synthetase activity. Glutamine in the pre ence of 2-oxoglutarate, stimulated nicotinamide nucleotide oxidation by crude and purified extracts of the strain and led to a reductant dependent formation of two molecules of glutamate. Aminooxyacetate did not have any effect on the reaction, whereas azaserine inhibited it completely. It is concluded that in glutamine synthetase and glutamate synthase are responsible for the assimilation of low ammonium concentrations. |
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Keywords: | AChR acetylcholine receptor |
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