Heme transport from rat liver mitochondria to the microsomes |
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Authors: | Shigeru Taketani Rikio Tokunaga |
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Institution: | Department of Hygiene, Kansai Medical University, Moriguchi, Osaka (570), Japan |
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Abstract: | When rat liver mitochondria labeled with 59Fe]heme were incubated with microsomes in the presence of cytosol, about 16 % of the heme in mitochondria was transported to microsomes during a 1 hr-incubation period. In the absence of cytosol, little heme was transported. DEAE-Sephadex column chromatography of the cytosol partially purified by pH 5.1 treatment and ammonium sulfate precipitation (45–65%) revealed that there were at least two proteins with a releasing activity from mitochondria via heme transport. |
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Keywords: | PMSF phenylmethylsulfonyl floride |
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