Detergent induced inhibition of eukaryotic RNA polymerase B activity and amanitin binding |
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| Authors: | Arnold Brown George M Garrity |
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| Institution: | 1. Department of Microbiology Graduate School of Public Health Pittsburgh, Pennsylvania 15240 USA;2. Department of Medicine U.S.V.A. Medical Center Pittsburgh, Pennsylvania 15240 USA;3. University of Pittsburgh Pittsburgh, Pennsylvania 15240 USA |
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| Abstract: | We studied the effect of detergents on the binding of amanitin to RNA polymerase and on enzymatic activity. SDoS, Sarkosyl and deoxycholate were most inhibitory. Cholate and non-ionic detergents were less inhibitory. Evidence is presented that Sarkosyl inhibits chain elongation. The inhibition of amanitin binding was most influenced by the hydrophilicity of the detergent. |
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