丁酸钠和pH值对离体蚕豆花药中小孢子有丝分裂的影响

含单核中后期至后期花粉的蚕豆离体花药,经过2mM丁酸钠24小时预处理后,再漂浮培养于pH5.8或7.0的液体培养基。以不经预处理的作为对照。结果如下: 1.培养后9天内,丁酸钠预处理和培养基pH值并不明显影响花粉退化百分率。2.培养初期,pH7.0显著促进小孢子不等分裂。3.丁酸钠预处理抑制培养初期的小孢子有丝分裂,而后又显著增加小孢子均等分裂百分率。4.丁酸钠预处理导致小孢子有丝分裂类型的趋向改变。本文还对丁酸钠导致有丝分裂类型趋向改变的可能原因,进行了讨论。

SODIUM BUTYRATE AND PH EFFECT ON THE MICROSPORE MITOSIS OF DETACHED VICIA FABA L.ANTHERS

Detached Vicia faba L.antherspretreated by 2mM sodium butyratefor 24 hours was floated on liquidMS medium containing 2mg/l 2,4-D with pH5.8 or 7.0.As com-pared with unpretreated anthers,theresults are found to be: 1. Within a 9 days-culture,pre-treatment of Na-butyrate and the pHvalue in medium do not obviously in-fluence the rate of degeneration ofmicrospores. 2. pH in medium adjusted to7.0 enhance significantly the mi-crospore mitosis. 3. Pretreatment of butyrate in-hibits significantly the mitosis and thenenhance significantly the symmetricmitosis. 4. Pretreatment of butyrate in-duces the alteration of the tendencyof mitotic type. The results suggest that the transient inhibition of mitosis by butyrateinduces the alteration of interrelationbetween anther wall and microspores,and/or induces directly the symmetricmitosis.