A new method of sample application for horizontal slab polyacrylamide gel electrophoresis |
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Authors: | Dale Every |
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Institution: | Wallaceville Animal Research Centre, Research Division, Ministry of Agriculture and Fisheries, Private Bag, Upper Hutt, New Zealand |
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Abstract: | A new method of sample application for horizontal slab polyacrylamide gel electrophoresis has been developed which solves the main problems associated with existing systems. A quick, simple procedure is described for placing a dry powder mixture of Celite and Sephadex into sample wells of any shape cut to the full depth of the gel slab. Samples can then be added to the powder to form a moist firm bed of material in the wells which prevents leakage of sample from the well. The method enables the quantitative electrophoresis of many samples with widely differing concentrations and volumes without the problems of electrodecantation, loss of electrical contact through the wells, or uneven penetration of sample through the full thickness of the gel. |
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