Application of high-performance liquid chromatography with a chemiluminescence detection system to determine catecholamines in urine |
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| Authors: | Shin-Ichiro Kobayashi Jun Sekino Kazumasa Honda Kazuhiro Imai |
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| Institution: | Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113, Japan |
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| Abstract: | Chemiluminescence generated with the reaction of bis(2,4,6-trichlorophenyl)oxalate and hydrogen peroxide was applied to a detection system for high-performance liquid chromatography to determine fluorescamine-labeled catecholamines. The sensitivity of the chemiluminescence detection system with 25 fmol of detection limit was approximately 20 times higher than that of a conventional fluorescence detection system. Norepinephrine and dopamine in human urine were determined by the use of the new high-performance liquid chromatography detection system with the coefficient of variation of less than 4.0%. Good correlations (r = 0.998 for norepinephrine and r = 0.999 for dopamine) were obtained between the values by the present method and the conventional method. |
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| Keywords: | To whom correspondence should be addressed |
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