High-performance liquid chromatography of proteins by gel permeation chromatography |
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| Authors: | Robert A Jenik John W Porter |
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| Institution: | 1. Lipid Metabolism Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53706 USA;2. the Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin 53706 USA |
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| Abstract: | The ability of two high-performance liquid chromatography gel permeation columns to separate proteins was evaluated. These columns gave satisfactory molecular weight separations for some, but not all, proteins tested. These results indicate that there are limitations in confidence of molecular weight determinations made by this technique. |
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