A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH |
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Authors: | John M. Thomas M.E. Hodes |
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Affiliation: | Department of Medical Genetics, Indiana University School of Medicine, Indianapolis, Indiana 46223 USA |
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Abstract: | A simple, discontinuous buffer system for polyacrylamide gel electrophoresis near neutral pH is described. The buffer is MOPS (3-[N-morpholine]propanesulfonic acid), the leading ion K+ and the trailing ion histidine. The system offers improved resolution of cationic proteins. |
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Keywords: | To whom all correspondence should be addressed. |
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