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A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH
Authors:John M. Thomas  M.E. Hodes
Affiliation:Department of Medical Genetics, Indiana University School of Medicine, Indianapolis, Indiana 46223 USA
Abstract:A simple, discontinuous buffer system for polyacrylamide gel electrophoresis near neutral pH is described. The buffer is MOPS (3-[N-morpholine]propanesulfonic acid), the leading ion K+ and the trailing ion histidine. The system offers improved resolution of cationic proteins.
Keywords:To whom all correspondence should be addressed.
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