Hemolytic assay for venom phospholipase A2 |
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| Authors: | Carl-Wilhelm Vogel Andreas Plückthun Hans J Müller-Eberhard Edward A Dennis |
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| Institution: | 1. Department of Molecular Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037 USA;2. Department of Chemistry, University of California at San Diego, La Jolla, California 92093 USA |
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| Abstract: | A rapid and sensitive spectrophotometric assay for venom phospholipase A2 based on the hemolysis of guinea pig erythrocytes in the presence of decomplemented serum and cardiotoxin (direct lytic factor) is described. This assay is particularly useful for rapid multisample analyses, such as those used in monitoring chromatography fractions, and is specific for phospholipase A2 in she presence of other potentially hemolytic venom components. The hemolytic mechanism is shown to be a combination of the action of lysophospholipids liberated from lipoproteins in the serum and the synergistic action of phospholipase A2 and cardiotoxin on the erythrocyte membrane. |
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