Evaluation of 3-hydroxy-3-methylglutaryl-CoA reductase activity by multiple-selected ion monitoring |
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| Authors: | G Cighetti E Santaniello G Galli |
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| Institution: | 1. Institute of Chemistry, School of Medicine, University of Milan, Milan, Italy;2. Institute of Pharmacology and Pharmacognosy, Laboratory of Applied Biochemistry, School of Pharmacy, University of Milan, Milan, Italy |
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| Abstract: | A new method for the evaluation of 3-hydroxy-3-methylglutaryl-CoA reductase activity is described, based on the multiple-selected ion monitoring of the amount of mevalonate formed in incubations of 3-hydroxy-3-methylglutaryl-CoA with microsomal proteins. Analysis is carried out on crude extracts using deuterated mevalonic acid lactone as internal standard. The sensitivity of the technique allows the quantitative evaluation of mevalonate in microassays (100 μg microsomal protein) of the enzyme activity at the minimum value of the diurnal rhythm. |
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