An improved high-pressure liquid chromatographic assay for 2-acetylaminofluorene and eight of its metabolites |
| |
| Authors: | Carole L Smith Snorri S Thorgeirsson |
| |
| Institution: | Biochemical Pharmacology Section, Laboratory of Chemical Pharmacology, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205 USA |
| |
| Abstract: | A high-pressure liquid chromatography method utilizing desferal mesylate as a solvent additive was developed for the simultaneous separation of 2-acetylaminofluorene, N-hydroxy-2-acetylaminofluorene, 9-hydroxy-2-acetylaminofluorene, 7-hydroxy-2-acetylaminofluorene, 5-hydroxy-2-acetylaminofluorene, 3-hydroxy-2-acetylaminofluorene, 1-hydroxy-2-acetylaminofluorene, 2-aminofluorene, and 2-acetylaminofluorene-9-one. The method was used to quantitate these metabolites formed when 2-acetylaminofluorene was incubated with freshly isolated rat liver cells, with rat liver microsomes or with microsomes prepared from isolated hepatocytes. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
