Separation of poly(ADP-ribosylated) nuclear proteins by polyacrylamide gel electrophoresis at acidic pH and low temperature |
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| Authors: | V.T. Dam G. Faribault G.G. Poirier |
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| Affiliation: | Unité de Recherche Gastro-Intestinale, Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, P. Q., J1K 2R1, Canada |
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| Abstract: | A method for the extraction and electrophoresis of poly(ADP-ribosylated) nuclear proteins is described. An extraction method using lithium dodecyl sulfate as detergent at pH 2.4 and room temperature is shown to fully extract nuclear proteins under conditions where full stability of protein-linked polymer is ensured. The polyacrylamide gel electrophoresis is performed again under conditions where full stability is ensured. This work provides a technique whereby misinterpretation of relative ADP ribosylation of nuclear proteins can be avoided. |
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| Keywords: | To whom correspondence and reprint requests should be sent. |
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