Measurement of aspartylglucosamine in physiological fluids with an amino acid analyzer: Fused peak analysis with dual photometers |
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| Authors: | Robert J. Carter Floyd F. Snyder |
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| Affiliation: | 1. Biochemistry Laboratory, Pediatric Research Centre, Alberta Children''s Hospital, Calgary, Alberta T2N 1N4, Canada;2. Division of Pediatrics, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 1N4, Canada;3. Division of Medical Biochemistry, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 1N4, Canada |
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| Abstract: | Methods for the quantitation of aspartylglucosamine using an amino acid analyzer equipped with a physiological column and dual photometers were developed. Aspartylglucosamine and urea coelute, but their ninhydrin derivatives have different color factors at 440 and 590 nm. The concentration of aspartylglucosamine may be determined in a urea mixture by amino acid chromatography, absolute peak area measurement, and the 590:440 peak area ratio. A second method was also developed in which urea was completely digested with urease before chromatography, permitting the direct quantitation of aspartylglucosamine. |
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| Keywords: | To whom correspondence should be addressed. |
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