Rapid purification of a prekallikrein from rat pancreas |
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| Authors: | Rebecca Matsas David Proud Kjell Nustad Graham S Bailey |
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| Institution: | Department of Chemistry, University of Essex, Colchester CO4 3SQ, Essex, United Kingdom |
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| Abstract: | A prekallikrein from rat pancreas was purified 1500-fold with an overall yield of 20% using a rapid, simple procedure. DEAE-Sephadex A-50 chromatography permitted the separation of two prekallikrein components present in rat pancreatic homogenates; the major fraction was further purified by Sephadex G-100 gel filtration and immunoadsorption chromatography. The zymogen is a single-chain molecule with pI 4·35. Apparent Mr values of 38,000 and 37,000 were determined by sodium dodecyl sulfate-polyacrylamide gradient electrophoresis and gel filtration, respectively. |
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