Ornithine decarboxylase (EC 4.1.1.17) assay based upon the retention of putrescine by a strong cation-exchange paper |
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Authors: | Rune Djurhuus |
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Affiliation: | Department of Biochemistry, University of Bergen, Årstadveien 19, 5000 Bergen, Norway |
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Abstract: | A simple and direct method for the detection of ornithine decarboxylase (EC 4.1.1.17) activity is presented. It is based upon the selective binding of putrescine to Whatman P81 paper, a strong cation-exchanger, in the presence of 0.1 m NH3. The assay is easy to perform and has an advantage over the more frequently used CO2 trapping methods which can yield spurious CO2 formation due to the action of enzymes other than ornithine decarboxylase. |
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