A radiometric microassay for cellulase activity |
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| Authors: | Alexander Vardanis Malcolm Finkelman |
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| Affiliation: | 1. London Research Centre, Agriculture Canada, London, Canada;2. Department of Microbiology and Immunology, The University of Western Ontario, London, Canada |
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| Abstract: | The method described measures the release of 14C products from [U-14C]cellulose. The assay is simple and utilizes very small incubation volumes. Activity is evident within the first 30 min of reaction. This method should be particularly suited to activity surveys of culture filtrates from fermentations as well as the analysis of fractions from enzyme purification studies. |
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