Rapid Quantitation of desmosine content in tissue hydrolysates by high-performance liquid chromatography |
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| Authors: | Barbara Faris Rocco Ferrera Michael Glembourtt Peter J. Mogayzel George Crombie Carl Franzblau |
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| Affiliation: | Department of Biochemistry, Boston University School of Medicine, 80 East Concord Street, Boston, Massachusetts 02118 USA |
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| Abstract: | The lysine-derived crosslinks in elastin, desmosine, and isodesmosine, are quantitated in tissue hydrolysates by monitoring high-performance liquid chromatography eluents at 275 nm. The results from this method compare favorably with results from the amino acid analyzer. However, this more sensitive method (1) eliminates ninhydrin-positive artifacts which elute with the desmosines from some tissue hydrolysates on the amino acid analyzer, and (2) makes possible elastin quantitation in a tissue with the minimum amount of manipulations. |
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